Metagenome@Kin measures the composition and diversity of microbial species in many environments including water, soil, microbiota, yogurt & probiotics, and beer. The wide range of its applications makes it a useful resource to countless fields including the food and medical industries. Reads taken from the next generation sequencer will be automatically annotated with taxon ID by BLAST or RDP Classifier, and grouped by taxonomic rank. Automatic clustering tools for metagenome research (PCA, HCL, SOM) are also included with the software.
Main Functions and Features
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Metagenomic analysis of bacteria to classify taxonomic ranks
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Fungal Metagenomic analysis from RDP Classifier results with ITS 1/2 sequence data
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Automatically create taxonomy tables from BLAST or RDP Classifier results
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User defined BLAST filter settings
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Confidence value filtering ability for the RDP Classifier results
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Taxonomic classification of bacteria follows the NCBI taxonomy rule
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All analyses can be performed through the user friendly GUI interfac
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16S/18S rRNA databases provided
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Exporting an HTML report is available to share result data
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Customizations available
Metagenonimc 16S/28S/ITS Data Analysis Software
Measures the Composition and Diversity of Microbial & Fungal Species from RNA-Seq Data
EASY TO USE • FAST • NO MANUAL COMMANDS!
Example of Metagenome@Kin Result
AUTOMATED, INTERACTIVE CHARTS & GRAPHS
Sunburst chart, pie chart, and bar chart are created automatically after dropping the files into Metagenome@Kin. Mouse over each section for microbe name and number of hit reads.
Multiple samples can be analyzed and displayed as a bar chart. The bar chart compares the microbial composition and diversity among various samples.
MULTIPLE SAMPLE ANALYSIS
ANALYZE EXCLUDED & REJECTED READS
You can include rejected hits - reads which did not not meet the filter conditions during the @Kin analysis stage, or reads excluded based on BLAST settings - and view them as graphs or clustering analysis.
Statistical analysis tools are automated in Metagenome@Kin, it generates heat clustering, 2D, 3D, and interactive PCA, and SOM plots.
STATISTICAL ANALYSIS TOOLS
DOWNLOAD a Sample Interactive Analysis Report
Unzip the file & open the "index.html" file in FireFox
SYSTEM REQUIREMENTS:
Hardware：
Windows 7/8/10 64bit, 16G RAM
Input data format:
Tab delimited BLAST result / RDP Classifier result
(QC/BLAST/RDP Classifier tools available upon request or Homology Analysis Tool)
Other software:
R 3.1 or later and Java 8 or later
* Metagenome@KIN trial version is a free tool and available. It provides taxonomic names and counts, pie graphs, sunburst graphs, and bar graphs up to the Genus rank. In order to download the trial version, click the button above.
Please note that your computer has at least Java 8 version.