Customizable LSKB Database Platform
LSKB database is customizable and integrable into your pipeline as your own search system.
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Over 50 collected database, linking with IDs
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Database resources from bioinformatics to cheminformatics
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Gene search from NGS, target search from compounds, or compound search from targets
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LSKB platform contains comprehensive search
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Database updates, 3 times yearly: no database collections and curation
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Provide query SQL
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Develop query pipeline script
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Support Pipeline Pilot by BIOVIA
For more details about registered database, please contact us.
LSKB Database Platform
WORLD FUSION US, Inc.
World Fusion
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Metagenome@Kin: 16S rRNA・ITS Metagenome Analysis Software
All analysis is completed on Windows
Software tool of Taxonomy ID annotations, collections, and statistical processes from a BLAST/RDP Classifier result
Metagenome@KIN is a 16S rRNA genome analytic tool which utilizes high-performance sequence data.
This tool executes grouping of bacterial species and hierarchical classification by drag-and-drop data, exported as a BLAST or RDP MultiClassifier result of read sequences, onto the World Fusion’s software.
Additionally, this tool enables users to compare the categorized result under each statistical feature. OTU function is also available.
Function List
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A list of Taxonomy IDs for each class from a BLAST or RDP Classifier result
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Metagenome analysis for bacteria or fungi
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Users can set up a filter, such as the number of mismatched bases in sequences from a BLAST result and the number of gap bases
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Once purchasing the software, it comes with a BLAST tool and a RDP Classifier without extra charges
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Confidence Value of a RDP Classifier result can be used as a filtering item
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Hierarchical classification based onTaxonomy database rules and classification through OTU feature
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A donut graph, pie graphs, and bar graphs will be automatically created
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Classified collection of a pie graph for each class
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Classified collection of a bar graph for each class
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The number of reads for each sample for each class
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Statistical analysis - SOM, PCA, PCoA, and hierarchical clustering
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Calculation of alpha diversity
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A result will be exported in a tab-separated text format
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Users can check a result without the software using an analysis result in a HTML report
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User friendly analysis operations
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16S rRNA database will be provided (contact us for other databases)
Pie chart or spread sheet for each class from species to Super kingdom
Bacterial species not found in Blast Database are also used for analysis
Pie chart or spread sheet for each class from species to Super kingdom
Specification
CPU:Core i5/ i7 or higher(8th generation or higher is recommended)
Memory:16Gb or higher is recommended
Storage: 1Tb or higher is recommended
O S : Windows 10 64bit
Need third party tools:
R 3.1 or later
Java 8 or later
License
Perpetual / PC
Analysis workflow
1. Sequence data
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Sequence data, supported for this software, is a Fastq file provided by Life Technologies Inc, Illumina Inc, or Oxford Nanopore Inc.
2. Pre-Process
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Reads, exported from a high performance sequencer, will undergo Quality Check, primer sequencing, adaptor sequencing, and Tag sequence trimming in advance.
3. Execution of BLAST/RDP MultiClassifier
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By using 16S rRNA database, users need to set up conditions on the BLAST execution window. If RDP MultiClassifier is necessary, the free tool will be provided along with the Blast program.
QC/Blast tool
・Pair-end merge
・QC Trimming
・Execution of BLAST/RDP MultiClassifier
・Classification through OTU feature
Step1. Determination of Taxonomy using QC/Blast tool
Step2
By drag-and-drop a BLAST result or a RDP MultiClassifier result onto the analysis result
4. Hierarhical Classification
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By drag-and-drop a BLAST result or a RDP MultiClassifier result onto the analysis result, automatically a list of NCBI Taxonomy ID annotations, a donut graph, pie graphs, and bar graphs for each sample, based on the hierarchical classification rules from the Taxonomy database, will be created.
5. Comparison Between Samples for Each Class.
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When multiple samples were analyzed, they can be comparable between samples using a pie graph or a bar graph for each class.
Statistical Process
Following statistical analysis will be automatically created to compare multiple samples from either a BLAST or a RDP Classifier result
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Principal Component Analysis (PCA) - 2D, 3D interactive, 3D annimation, PCoA
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Self-organization map (SOM)
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Hierarchical clustering
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α diversity calculation
Trial
We provide a 2-weeks trial of this program.
Fill out the form, then we will send download link.
Metagenome@KIN_J.Zip (424.7 MB)
Specification
CPU:Core i5/ i7 or higher(8th generation or higher is recommended)
Memory:16Gb or higher is recommended
Storage: 1Tb or higher is recommended
O S : Windows 10 64bit
Need third party tools:
R 3.1 or later
Java 8 or later