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Automated Identification Tool of Influenza HA・NA subtypes from a sequencer

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Compatible with sequencers from Illumina MiSeq and Oxford Nanopore

All analysis is completed on Windows

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FluGAS: Automatic Influenza Genome Assembly and subtyping

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FluGas is a Windows software which can identify a subtype and base sequences for each segment from influenza virus genome sequence data with easy operations through a high-performance sequencer. 

It automatically assembles whole genome sequence data, which is exported with enormous data of Influenza type A and B from a sequencer. ​

  • Automatic identification of an influenza subtype directly from a Fastq file (Illumina MiSeq) or a Fast5 file (Nanopore DNA sequencer)

  • Automatically assemble base sequences for each segment and construct consensus sequences for all eight segments

  • User friendly operation of Fastq files and compatible with a batch process for multiple samples

  • Compatible with both influenza A and B types

  • For the A type influenza, identify the subtype of HA or NA while for the B type, identify Victoria or Yamagata.

  • Database used for the identification of subtypes is referred to by registered sequences from NCBI Influenza virus resources. 

  • Analysis results will be exported as an excel file in a GISAID format, and results with multiple samples will be exported as a tab-separated text file format. 

  • Analysis results will be saved in the application, and it is easy to see the results by assigning the analysis date. 

  • Compatible with all animal derived influenza, such as birds, ligs, horses, and humans.

  • Feature of database updates

Sequencer setting
Sequencer setting

Drag and drop Fastq files from Illumina MiSeq, or Fast5 files from Oxford Nanopore DNA Sequencer

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Analysis setting
Analysis setting

Setting for Quality Control of Read data, mapping and identify a subtype

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Export GISAID
Export GISAID

An identification result will be exported as a public database  GISAID excel file format

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Sequencer setting
Sequencer setting

Drag and drop Fastq files from Illumina MiSeq, or Fast5 files from Oxford Nanopore DNA Sequencer

press to zoom
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Specification

CPU:Core i5/ i7 or higher(8th generation or higher is recommended)

Memory:8Gb(16Gb or higher is recommended)

Storage: 1Tb or higher is recommended(Install storage 500Gb)

O  S   : Windows 10/11 64bit (version 1709 or later)

License

Perpetual / PC

Analysis workflow
1. Sequence data
  • Drag-and-drop a Fastq(Miseq) file or folder(Nanopore DNA sequence) (multiple files or folders can be selected)

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An image of entering sequence data. Select either MiSeq or MinION and drag-and-drop sequence data.

2. QC check (self-executing)
  • Quality Control of Read data (trimming based on Phread score and the Read length)

3. Mapping (self-executing)
  • After constructing a consensus sequence from a mapping results, identify a subtype.

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4.  Identification of Whole Genome Sequence and a Subtype (self-executing)
  • Assembling whole segments of influenza virus and mapping for identification of a subtype

4.  Report
  • An identification result will be exported as a public database  GISAID excel file format.

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  • Consensus sequences for each segment will also be exported in a Fasta format.

*In order to analyze a Fast5 file, exported from a Nanopore DNA sequencer, you will need software, provided by Oxford Nanopore.

World Fusion Co., LTD is authorized to develop and sell this software (FluGAS) based on an accomplishment of the collaboration research with National Research and Development Agency, the National Agriculture and Food Research Organization, the department of the national institute of Animal Health.

Trial

We provide a 2-weeks trial of this program. 

Fill out the form, then we will send download link. 

Flugasv2.Zip (152.5MB)